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Journal of Biomedical Optics • Open Access

In planta imaging of Δ9-tetrahydrocannabinolic acid in Cannabis sativa L. with hyperspectral coherent anti-Stokes Raman scattering microscopy
Author(s): Erik T. Garbacik; Roza P. Korai; Eric H. Frater; Jeroen P. Korterik; Cees Otto; Herman L. Offerhaus

Paper Abstract

Nature has developed many pathways to produce medicinal products of extraordinary potency and specificity with significantly higher efficiencies than current synthetic methods can achieve. Identification of these mechanisms and their precise locations within plants could substantially increase the yield of a number of natural pharmaceutics. We report label-free imaging of Δ 9 -tetrahydrocannabinolic acid (THCa) in Cannabis sativa L. using coherent anti-Stokes Raman scattering microscopy. In line with previous observations we find high concentrations of THCa in pistillate flowering bodies and relatively low amounts within flowering bracts. Surprisingly, we find differences in the local morphologies of the THCa-containing bodies: organelles within bracts are large, diffuse, and spheroidal, whereas in pistillate flowers they are generally compact, dense, and have heterogeneous structures. We have also identified two distinct vibrational signatures associated with THCa, both in pure crystalline form and within Cannabis plants; at present the exact natures of these spectra remain an open question.

Paper Details

Date Published: 15 April 2013
PDF: 6 pages
J. Biomed. Opt. 18(4) 046009 doi: 10.1117/1.JBO.18.4.046009
Published in: Journal of Biomedical Optics Volume 18, Issue 4
Show Author Affiliations
Erik T. Garbacik, Univ. Twente (Netherlands)
Roza P. Korai, Univ. Twente (Netherlands)
Eric H. Frater, Univ. Twente (Netherlands)
Jeroen P. Korterik, Univ. Twente (Netherlands)
Cees Otto, Univ. Twente (Netherlands)
Herman L. Offerhaus, Univ. Twente (Netherlands)


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