Share Email Print

Journal of Biomedical Optics

Rapid quantification of pixel-wise fiber orientation data in micrographs
Author(s): Kyle P. Quinn; Irene Georgakoudi
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Defining fiber orientation at each pixel within a medical image has traditionally been computationally intensive and prone to systematic errors. A weighted orientation vector summation algorithm capable of detecting fiber orientation simultaneously at each pixel within an image is presented. As a result, pixel-specific fiber orientation information with 2 deg to 3 deg accuracy can be determined within seconds, enabling the practical use of pixel-wise orientation data for characterizing structural anisotropy. This analysis technique has applicability and potential diagnostic utility for a variety of modalities, including second harmonic generation, scanning electron microscopy and immunohistochemical imaging is demonstrated.

Paper Details

Date Published: 1 April 2013
PDF: 8 pages
J. Biomed. Opt. 18(4) 046003 doi: 10.1117/1.JBO.18.4.046003
Published in: Journal of Biomedical Optics Volume 18, Issue 4
Show Author Affiliations
Kyle P. Quinn, Tufts Univ. (United States)
Irene Georgakoudi, Tufts Univ. (United States)

© SPIE. Terms of Use
Back to Top