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Journal of Biomedical Optics • Open Access

Purple sea urchin Strongylocentrotus purpuratus gamete manipulation using optical trapping and microfluidics
Author(s): Charlie Chandsawangbhuwana; Linda Z. Shi; Qingyuan Zhu; Michael W. Berns

Paper Abstract

A system has been developed that allows for optical and fluidic manipulation of gametes. The optical manipulation is performed by using a single-point gradient trap with a 40× oil immersion PH3 1.3 NA objective on a Zeiss inverted microscope. The fluidic manipulation is performed by using a custom microfluidic chamber designed to fit into the short working distance between the condenser and objective. The system is validated using purple sea urchin Strongylocentrotus purpuratus gametes and has the potential to be used for mammalian in vitro fertilization and animal husbandry.

Paper Details

Date Published: 22 March 2013
PDF: 4 pages
J. Biomed. Opt. 18(4) 040501 doi: 10.1117/1.JBO.18.4.040501
Published in: Journal of Biomedical Optics Volume 18, Issue 4
Show Author Affiliations
Charlie Chandsawangbhuwana, Univ. of California, San Diego (United States)
Linda Z. Shi, Univ. of California, San Diego (United States)
Qingyuan Zhu, Univ. of California, San Diego (United States)
Michael W. Berns, Univ. of California, San Diego (United States)


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