Journal of Biomedical Optics • Open AccessRapid quantification of pixel-wise fiber orientation data in micrographs
Defining fiber orientation at each pixel within a medical image has traditionally been computationally intensive and prone to systematic errors. A weighted orientation vector summation algorithm capable of detecting fiber orientation simultaneously at each pixel within an image is presented. As a result, pixel-specific fiber orientation information with 2 deg to 3 deg accuracy can be determined within seconds, enabling the practical use of pixel-wise orientation data for characterizing structural anisotropy. This analysis technique has applicability and potential diagnostic utility for a variety of modalities, including second harmonic generation, scanning electron microscopy and immunohistochemical imaging is demonstrated.