Share Email Print

Journal of Biomedical Optics • Open Access

Rapid quantification of pixel-wise fiber orientation data in micrographs
Author(s): Kyle P. Quinn; Irene Georgakoudi

Paper Abstract

Defining fiber orientation at each pixel within a medical image has traditionally been computationally intensive and prone to systematic errors. A weighted orientation vector summation algorithm capable of detecting fiber orientation simultaneously at each pixel within an image is presented. As a result, pixel-specific fiber orientation information with 2 deg to 3 deg accuracy can be determined within seconds, enabling the practical use of pixel-wise orientation data for characterizing structural anisotropy. This analysis technique has applicability and potential diagnostic utility for a variety of modalities, including second harmonic generation, scanning electron microscopy and immunohistochemical imaging is demonstrated.

Paper Details

PDF: 8 pages
J. Biomed. Opt. 18(4) 040102 doi: 10.1117/1.JBO.18.4.040102
Published in: Journal of Biomedical Optics Volume 18, Issue 4, April 2013
Show Author Affiliations
Kyle P. Quinn, Tufts Univ. (United States)
Irene Georgakoudi, Tufts Univ. (United States)

© SPIE. Terms of Use
Back to Top