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Journal of Biomedical Optics • Open Access

Effects of low-level laser therapy on the expression of osteogenic genes related in the initial stages of bone defects in rats
Author(s): Kelly R. Fernandes; Daniel A. Ribeiro; Natalia C. Rodrigues; Carla Tim; Anderson A. Santos; Nivaldo A. Parizotto; Helosia S. de Araujo; Patricia Driusso; Ana Claudia Renno

Paper Abstract

We evaluate the effects of low-level laser therapy (LLLT) on the histological modifications and temporal osteogenic genes expression during the initial phase of bone healing in a model of bone defect in rats. Sixty-four Wistar rats were divided into control and treated groups. Noncritical size bone defects were surgically created at the upper third of the tibia. Laser irradiation (Ga-Al-As laser 830 nm, 30 mW, 0.028  cm 2 , 1.071  W/cm 2 , 1 min and 34 s, 2.8 Joules, 100  J/cm 2 ) was performed for 1, 2, 3, and 5 sessions. Histopathology revealed that treated animals presented higher inflammatory cells recruitment, especially 12 and 36 h postsurgery. Also, a better tissue organization at the site of the injury, with the presence of granulation tissue and new bone formation was observed on days three and five postsurgery in the treated animals. The quantitative real time polymerase chain reaction showed that LLLT produced a significantly increase in mRNA expression of Runx-2, 12 h and three days post-surgery, a significant upregulation of alkaline phosphatase mRNA expression after 36 h and three days post-surgery and a significant increase of osteocalcin mRNA expression after three and five days. We concluded that LLLT modulated the inflammatory process and accelerated bone repair, and this advanced repair pattern in the laser-treated groups may be related to the higher mRNA expression of genes presented by these animals.

Paper Details

Date Published: 20 March 2013
PDF: 7 pages
J. Biomed. Opt. 18(3) 038002 doi: 10.1117/1.JBO.18.3.038002
Published in: Journal of Biomedical Optics Volume 18, Issue 3
Show Author Affiliations
Kelly R. Fernandes, Univ. Federal de São Paulo (Brazil)
Daniel A. Ribeiro, Univ. Federal de São Paulo (Brazil)
Natalia C. Rodrigues, Univ. Federal de São Carlos (Brazil)
Carla Tim, Univ. Federal de São Paulo (Brazil)
Anderson A. Santos, Univ. Federal de São Paulo (Brazil)
Nivaldo A. Parizotto, Univ. Federal de São Paulo (Brazil)
Helosia S. de Araujo, Univ. Federal de São Paulo (Brazil)
Patricia Driusso, Univ. Federal de São Paulo (Brazil)
Ana Claudia Renno, Univ. Federal de São Paulo (Brazil)

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