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Journal of Biomedical Optics

Spectroscopic imaging of serum proteins using quantum cascade lasers
Author(s): Anadi Mukherjee; Quentin Bylund; Manu Prasanna; Yotam Margalit; Tarik Tihan
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Paper Abstract

First measurements of biomedical imaging using quantum cascade lasers (QCL) are presented. We report spectroscopic imaging of serum proteins using QCLs as an example for monitoring surface biocontamination. We found that dry smears of human serum can be spectroscopically imaged, identified, and quantified with high sensitivity and specificity. The core parts of the imaging platform consist of optically multiplexing three QCLs and an uncooled microbolometer camera. We show imaging of human serum proteins at 6.1, 9.25, and 9.5 μm QCLs with high sensitivity and specificity. The sensitivity limit of 3  μg/cm 2 of the human serum spot was measured at an S/N=3 .The specificity of human serum detection was measured at 99% probability at a threshold of 77  μg/cm 2 . We anticipate our imaging technique to be a starting point for more sophisticated biomolecular diagnostic applications.

Paper Details

Date Published: 20 March 2013
PDF: 5 pages
J. Biomed. Opt. 18(3) 036011 doi: 10.1117/1.JBO.18.3.036011
Published in: Journal of Biomedical Optics Volume 18, Issue 3
Show Author Affiliations
Anadi Mukherjee, Infrasign LLC (United States)
Quentin Bylund, Infrasign LLC (United States)
Manu Prasanna, Infrasign LLC (United States)
Yotam Margalit, Infrasign LLC (United States)
Tarik Tihan, Infrasign LLC (United States)

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