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Journal of Biomedical Optics • Open Access

Direct trabecular meshwork imaging in porcine eyes through multiphoton gonioscopy
Author(s): Omid Masihzadeh; David A. Ammar; Malik Y. Kahook; Emily A. Gibson; Tim C. Lei

Paper Abstract

The development of technologies to characterize the ocular aqueous outflow system (AOS) is important for the understanding of the pathophysiology of glaucoma. Multiphoton microscopy (MPM) offers the advantage of high-resolution, label-free imaging with intrinsic image contrast because the emitted signals result from the specific biomolecular content of the tissue. Previous attempts to use MPM to image the murine irido-corneal region directly through the sclera have suffered from degradation in image resolution due to scattering of the focused laser light. As a result, transscleral MPM has limited ability to observe fine structures in the AOS. In this work, the porcine irido-corneal angle was successfully imaged through the transparent cornea using a gonioscopic lens to circumvent the highly scattering scleral tissue. The resulting high-resolution images allowed the detailed structures in the trabecular meshwork (TM) to be observed. Multimodal imaging by two-photon autofluorescence and second harmonic generation allowed visualization of different features in the TM without labels and without disruption of the TM or surrounding tissues. MPM gonioscopy is a promising noninvasive imaging tool for high-resolution studies of the AOS, and research continues to explore the potential for future clinical applications in humans.

Paper Details

Date Published: 20 March 2013
PDF: 6 pages
J. Biomed. Opt. 18(3) 036009 doi: 10.1117/1.JBO.18.3.036009
Published in: Journal of Biomedical Optics Volume 18, Issue 3
Show Author Affiliations
Omid Masihzadeh, Univ. of Colorado Denver School of Medicine (United States)
David A. Ammar, Univ. of Colorado Denver School of Medicine (United States)
Malik Y. Kahook, Univ. of Colorado Denver School of Medicine (United States)
Emily A. Gibson, Univ. of Colorado Denver (United States)
Tim C. Lei, Univ. of Colorado Denver (United States)


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