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Journal of Biomedical Optics • Open Access

Staining-free malaria diagnostics by multispectral and multimodality light-emitting-diode microscopy
Author(s): Aboma J. Merdasa; Mikkel Brydegaard; Sune Svanberg; Jeremie T. Zoueu

Paper Abstract

We report an accurate optical differentiation technique between healthy and malaria-infected erythrocytes by quasi-simultaneous measurements of transmittance, reflectance, and scattering properties of unstained blood smears using a multispectral and multimode light-emitting diode microscope. We propose a technique for automated imaging, identification, and counting of malaria-infected erythrocytes for real-time and cost-effective parasitaemia diagnosis as an effective alternative to the manual screening of stained blood smears, now considered to be the gold standard in malaria diagnosis. We evaluate the performance of our algorithm against manual estimations of an expert and show a spectrally resolved increased scattering from malaria-infected blood cells.

Paper Details

Date Published: 1 March 2013
PDF: 11 pages
J. Biomed. Opt. 18(3) 036002 doi: 10.1117/1.JBO.18.3.036002
Published in: Journal of Biomedical Optics Volume 18, Issue 3
Show Author Affiliations
Aboma J. Merdasa, Lund Univ. (Sweden)
Mikkel Brydegaard, Lund Univ. (Sweden)
Sune Svanberg, Lund Univ. (Sweden)
Jeremie T. Zoueu, Lab. d'Instrumentation Image et Spectroscopy (Cote d Ivoire)

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