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Journal of Biomedical Optics • Open Access

Large area periodic, systematically changing, multishape nanostructures by laser interference lithography and cell response to these topographies
Author(s): Erden Ertorer; Fartash Vasefi; Joel Keshwah; Mohamadreza Najiminaini; Christopher Halfpap; Uwe Langbein; Jeffrey J. L. Carson; Douglas W. Hamilton; Silvia Mittler

Paper Abstract

The fabrication details to form large area systematically changing multishape nanoscale structures on a chip by laser interference lithography (LIL) are described. The feasibility of fabricating different geometries including dots, ellipses, holes, and elliptical holes in both x - and y - directions on a single substrate is shown by implementing a Lloyd’s interferometer. The fabricated structures at different substrate positions with respect to exposure time, exposure angle and associated light intensity profile are analyzed. Experimental details related to the fabrication of symmetric and biaxial periodic nanostructures on photoresist, silicon surfaces, and ion milled glass substrates are presented. Primary rat calvarial osteoblasts were grown on ion-milled glass substrates with nanotopography with a periodicity of 1200 nm. Fluorescent microscopy revealed that cells formed adhesions sites coincident with the nanotopography after 24 h of growth on the substrates. The results suggest that laser LIL is an easy and inexpensive method to fabricate systematically changing nanostructures for cell adhesion studies. The effect of the different periodicities and transition structures can be studied on a single substrate to reduce the number of samples significantly.

Paper Details

Date Published: 4 March 2013
PDF: 9 pages
J. Biomed. Opt. 18(3) 035002 doi: 10.1117/1.JBO.18.3.035002
Published in: Journal of Biomedical Optics Volume 18, Issue 3
Show Author Affiliations
Erden Ertorer, The Univ. of Western Ontario (Canada)
Fartash Vasefi, Lawson Health Research Institute (Canada)
Joel Keshwah, The Univ. of Western Ontario (Canada)
Mohamadreza Najiminaini, Lawson Health Research Institute (Canada)
Christopher Halfpap, Fachhochschule Wiesbaden (Germany)
Uwe Langbein, Fachhochschule Wiesbaden (Germany)
Jeffrey J. L. Carson, Lawson Health Research Institute (Canada)
Douglas W. Hamilton, The Univ. of Western Ontario (Canada)
Silvia Mittler, The Univ. of Western Ontario (Canada)

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