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Journal of Biomedical Optics

In vivo observation of age-related structural changes of dermal collagen in human facial skin using collagen-sensitive second harmonic generation microscope equipped with 1250-nm mode-locked Cr:Forsterite laser
Author(s): Takeshi Yasui; Makoto Yonetsu; Ryosuke Tanaka; Yuji Tanaka; Shu-ichiro Fukushima; Toyonobu Yamashita; Yuki Ogura; Tetsuji Hirao; Hiroyuki Murota; Tsutomu Araki
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Paper Abstract

In vivo visualization of human skin aging is demonstrated using a Cr:Forsterite (Cr:F) laser-based, collagen-sensitive second harmonic generation (SHG) microscope. The deep penetration into human skin, as well as the specific sensitivity to collagen molecules, achieved by this microscope enables us to clearly visualize age-related structural changes of collagen fiber in the reticular dermis. Here we investigated intrinsic aging and/or photoaging in the male facial skin. Young subjects show dense distributions of thin collagen fibers, whereas elderly subjects show coarse distributions of thick collagen fibers. Furthermore, a comparison of SHG images between young and elderly subjects with and without a recent life history of excessive sun exposure show that a combination of photoaging with intrinsic aging significantly accelerates skin aging. We also perform image analysis based on two-dimensional Fourier transformation of the SHG images and extracted an aging parameter for human skin. The in vivo collagen-sensitive SHG microscope will be a powerful tool in fields such as cosmeceutical sciences and anti-aging dermatology.

Paper Details

Date Published: 5 December 2012
PDF: 11 pages
J. Biomed. Opt. 18(3) 031108 doi: 10.1117/1.JBO.18.3.031108
Published in: Journal of Biomedical Optics Volume 18, Issue 3
Show Author Affiliations
Takeshi Yasui, Osaka Univ. (Japan)
Makoto Yonetsu, Osaka Univ. (Japan)
Ryosuke Tanaka, Osaka Univ. (Japan)
Yuji Tanaka, Osaka Univ. (Japan)
Shu-ichiro Fukushima, Osaka Univ. (Japan)
Toyonobu Yamashita, Shiseido Co., Ltd. (Japan)
Yuki Ogura, Shiseido Co., Ltd. (Japan)
Tetsuji Hirao, Shiseido Co., Ltd. (Japan)
Hiroyuki Murota, Osaka Univ. Graduate School of Medicine, FOM (Japan)
Tsutomu Araki, Osaka Univ. (Japan)

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