Share Email Print

Journal of Biomedical Optics

Second harmonic generation reveals matrix alterations during breast tumor progression
Author(s): Kathleen Burke; Ping Tang; Edward B. Brown
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Alteration of the extracellular matrix in tumor stroma influences efficiency of cell locomotion away from the primary tumor into surrounding tissues and vasculature, thereby affecting metastatic potential. We study matrix changes in breast cancer through the use of second harmonic generation (SHG) of collagen in order to improve the current understanding of breast tumor stromal development. Specifically, we utilize a quantitative analysis of the ratio of forward to backward propagating SHG signal (F/B ratio) to monitor collagen throughout ductal and lobular carcinoma development. After detection of a significant decrease in the F/B ratio of invasive but not in situ ductal carcinoma compared with healthy tissue, the collagen F/B ratio is investigated to determine the evolution of fibrillar collagen changes throughout tumor progression. Results are compared with the progression of lobular carcinoma, whose F/B signature also underwent significant evolution during progression, albeit in a different manner, which offers insight into varying methods of tissue penetration and collagen manipulation between the carcinomas. This research provides insights into trends of stromal reorganization throughout breast tumor development.

Paper Details

Date Published: 22 November 2012
PDF: 10 pages
J. Biomed. Opt. 18(3) 031106 doi: 10.1117/1.JBO.18.3.031106
Published in: Journal of Biomedical Optics Volume 18, Issue 3
Show Author Affiliations
Kathleen Burke, Univ. of Rochester (United States)
Ping Tang, Univ. of Rochester Medical Ctr. (United States)
Edward B. Brown, Univ. of Rochester (United States)

© SPIE. Terms of Use
Back to Top