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Journal of Biomedical Optics

Analysis of collagen fiber domain organization by Fourier second harmonic generation microscopy
Author(s): Ara Ghazaryan; Halley F. Tsai; Gor Hayrapetyan; Wei-Liang Chen; Yang-Fang Chen; Myung-Yung Jeong; Chang-Seok Kim; Shean-Jen Chen; Chen-Yuan Dong
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Paper Abstract

We present an automated and systematic two-dimensional discrete Fourier transform (2D-FFT) approach to analyze collagen fiber organization through the use of second harmonic generation (SHG) microscopy. Average orientations of individual domains and Ising-like order parameters introduced to characterize the correlation between orientations of adjacent domains may be used to quantitatively characterize fibrous tissues. Our approach was applied to analyze tissues including rat tail tendon, mouse skin, bovine corneas, and human corneas. We also show that collagen fiber organization in normal and keratokonus human corneas may be distinguished. The current approach may be used for the quantitative differentiation of SHG collagen fiber morphology in different tissues and may be applied for diagnostic purposes.

Paper Details

Date Published: 22 November 2012
PDF: 6 pages
J. Biomed. Opt. 18(3) 031105 doi: 10.1117/1.JBO.18.3.031105
Published in: Journal of Biomedical Optics Volume 18, Issue 3
Show Author Affiliations
Ara Ghazaryan, National Taiwan Univ. (Taiwan)
Halley F. Tsai, National Taiwan Univ. (Taiwan)
Gor Hayrapetyan, Yerevan State Univ. (Armenia)
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Yang-Fang Chen, National Taiwan Univ. (Taiwan)
Myung-Yung Jeong, Pusan National Univ. (Korea, Republic of)
Chang-Seok Kim, Pusan National Univ. (Korea, Republic of)
Shean-Jen Chen, National Cheng Kung Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

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