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Journal of Biomedical Optics

Study of molecule variations in renal tumor based on confocal micro-Raman spectroscopy
Author(s): Zhengfei Zhuang; Ning Li; Zhouyi Guo; Meifang Zhu; Ke Xiong; Sijin Chen
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Paper Abstract

Confocal micro-Raman spectroscopy-a valuable analytical tool in biological and medical field of research-allows probing molecular vibrations of samples without external labels or extensive preparation. We employ confocal micro-Raman spectroscopy to characterize renal tumors and normal tissue. Results show that Raman peaks of the renal tumor at 788 and 1087  cm−1, which belong to νsPO2− and νasPO2− stretching, respectively, have an obvious increase. At the same time, the ratio of I855/I831 in renal tumor tissue is 1.39±0.08, while that in normal renal tissue is 2.44±0.05 (p<0.01). This means that more tyrosine conformation transform from "buried" to "exposed" in the presence of cancer. Principal component analysis is used to classify the Raman spectra of renal tumor tissue and normal tissue.

Paper Details

Date Published: 22 November 2012
PDF: 6 pages
J. Biomed. Opt. 18(3) 031103 doi: 10.1117/1.JBO.18.3.031103
Published in: Journal of Biomedical Optics Volume 18, Issue 3
Show Author Affiliations
Zhengfei Zhuang, South China Normal Univ. (China)
Ning Li, South China Normal Univ. (China)
Zhouyi Guo, South China Normal Univ. (China)
Meifang Zhu, South China Normal Univ. (China)
Ke Xiong, Southern Medical Univ. (China)
Sijin Chen, Southern Medical Univ. (China)


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