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Journal of Biomedical Optics • Open Access

Development of a multiplexing fingerprint and high wavenumber Raman spectroscopy technique for real-time in vivo tissue Raman measurements at endoscopy
Author(s): Mads Sylvest Bergholt; Wei Zheng; Zhiwei Huang

Paper Abstract

We report on the development of a novel multiplexing Raman spectroscopy technique using a single laser light together with a volume phase holographic (VPH) grating that simultaneously acquires both fingerprint (FP) and high wavenumber (HW) tissue Raman spectra at endoscopy. We utilize a customized VPH dual-transmission grating, which disperses the incident Raman scattered light vertically onto two separate segments (i.e., −150 to 1950  cm −[sup]1 ; 1750 to 3600  cm −[sup]1 ) of a charge-coupled device camera. We demonstrate that the multiplexing Raman technique can acquire high quality in vivo tissue Raman spectra ranging from 800 to 3600  cm 1 within 1.0 s with a spectral resolution of 3 to 6  cm −1 during clinical endoscopy. The rapid multiplexing Raman spectroscopy technique covering both FP and HW ranges developed in this work has potential for improving in vivo tissue diagnosis and characterization at endoscopy.

Paper Details

Date Published: 28 February 2013
PDF: 4 pages
J. Biomed. Opt. 18(3) 030502 doi: 10.1117/1.JBO.18.3.030502
Published in: Journal of Biomedical Optics Volume 18, Issue 3
Show Author Affiliations
Mads Sylvest Bergholt, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)


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