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Journal of Biomedical Optics • Open Access

Reflection-mode multifocal optical-resolution photoacoustic microscopy

Paper Abstract

Compared with single-focus optical-resolution photoacoustic microscopy (OR-PAM), multifocal OR-PAM utilizes both multifocal optical illumination and an ultrasonic array transducer, significantly increasing the imaging speed. A reflection-mode multifocal OR-PAM system based on a microlens array that provides multiple foci as well as an ultrasonic array transducer that receives the excited photoacoustic waves from all foci simultaneously is presented. Using a customized microprism to reflect the incident laser beam to the microlens array, the multiple optical foci are aligned confocally with the focal zone of the ultrasonic array transducer. Experiments show the reflection-mode multifocal OR-PAM is capable of imaging microvessels in vivo, and it can image a 6×5×2.5  mm 3 volume at 16 μm lateral resolution in ∼2.5  min , which was limited by the signal multiplexing ratio and laser pulse repetition rate.

Paper Details

Date Published: 27 February 2013
PDF: 4 pages
J. Biomed. Opt. 18(3) 030501 doi: 10.1117/1.JBO.18.3.030501
Published in: Journal of Biomedical Optics Volume 18, Issue 3
Show Author Affiliations
Guo Li, Washington Univ. in St. Louis (United States)
Konstantin I. Maslov, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)

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