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Journal of Biomedical Optics • Open Access

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition
Author(s): Barry R. Masters

Paper Abstract

The second edition of Fundamentals of Light Microscopy and Electronic Imaging is a collaborative effort of Douglas B. Murphy and Michael W. Davidson. Murphy is known for his establishment of the School of Medicine Microscope Facility at the Johns Hopkins School of Medicine. He directed the facility until 2006 and has superb experience as a teacher of microscopy, optics, and image processing. He was the sole author of the first edition of this text and reference book. Davidson, who is at the National High Magnetic Field Laboratory, The Florida State University, is well known for his development of websites for online learning of microscopy; especially his development of dynamic Java and Flash tutorials to illustrate the principles and the applications. Davidson and his collaborators have produced very useful dynamic Java and Flash tutorials which are highly recommended. Nikon supports the site: CrossRef


. Olympus supports the Microscopy Resource Center at CrossRef


. I have used both of these education sites, and I highly recommend them to the readers of the Journal of Biomedical Optics. The combined work of these two authors results in a recommended textbook and reference that is an excellent starting point for someone new to the field of microscopy. While there is much information contained within the covers, from theory to applications, to demonstrations that the reader can perform in the laboratory, to discussions of limitations, it must be stated that this book is directed to the novice in microscopy and is an entry-level exposition of the fundamentals of the subject, devoid of the mathematics that is usual for optics books. With that disclaimer, I think that this highly acclaimed book is well-suited to the person who is attempting to learn the fundamentals of microscopy and electronic imaging.

Paper Details

Date Published: 14 February 2013
PDF: 2 pages
J. Biomed. Opt. 18(2) 029901 doi: 10.1117/1.JBO.18.2.029901
Published in: Journal of Biomedical Optics Volume 18, Issue 2
Show Author Affiliations
Barry R. Masters, Massachusetts Institute of Technology (United States)

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