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Journal of Biomedical Optics

In vivo detection of epileptic brain tissue using static fluorescence and diffuse reflectance spectroscopy
Author(s): Nitin Yadav; Sanjiv Bhatia; John Ragheb; Rupal Mehta; Prasanna Jayakar; William H. Yong; Wei-Chiang Lin
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Paper Abstract

Diffuse reflectance and fluorescence spectroscopy are used to detect histopathological abnormalities of an epileptic brain in a human subject study. Static diffuse reflectance and fluorescence spectra are acquired from normal and epileptic brain areas, defined by electrocorticography (ECoG), from pediatric patients undergoing epilepsy surgery. Biopsy specimens are taken from the investigated sites within an abnormal brain. Spectral analysis reveals significant differences in diffuse reflectance spectra and the ratio of fluorescence and diffuse reflectance spectra from normal and epileptic brain areas defined by ECoG and histology. Using these spectral differences, tissue classification models with accuracy above 80% are developed based on linear discriminant analysis. The differences between the diffuse reflectance spectra from the normal and epileptic brain areas observed in this study are attributed to alterations in the static hemodynamic characteristics of an epileptic brain, suggesting a unique association between the histopathological and the hemodynamic abnormalities in an epileptic brain.

Paper Details

Date Published: 5 February 2013
PDF: 8 pages
J. Biomed. Opt. 18(2) 027006 doi: 10.1117/1.JBO.18.2.027006
Published in: Journal of Biomedical Optics Volume 18, Issue 2
Show Author Affiliations
Nitin Yadav, Florida International Univ. (United States)
Sanjiv Bhatia, Miami Children's Hospital (United States)
John Ragheb, Miami Children's Hospital (United States)
Rupal Mehta, Univ. of California, Los Angeles (United States)
Prasanna Jayakar, Miami Children's Hospital (United States)
William H. Yong, Univ. of California, Los Angeles (United States)
Wei-Chiang Lin, Florida International Univ. (United States)


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