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Journal of Biomedical Optics • Open Access

Micro-Raman spectroscopy study of cancerous and normal nasopharyngeal tissues
Author(s): Yongzeng Li; Jianji Pan; Guannan Chen; Chao Li; Shaojun Lin; Yonghong Shao; Shangyuan Feng; Zufang Huang; Shusen Xie; Haishan Zeng; Rong Chen

Paper Abstract

The capabilities of micro-Raman spectroscopy for differentiating normal and malignant nasopharyngeal tissues were evaluated. Raman scattering signals were acquired from 22 normal and 52 malignant nasopharyngeal tissue samples. Distinctive spectral differences in Raman spectra between normal and malignant nasopharyngeal tissues were found, particularly in the spectral ranges of 853, 937, 1094, 1209, 1268, 1290 to 1340, 1579, and 1660  cm −1 , which primarily contain signals related to proteins, DNA, and lipids. Compared to normal tissues, the band intensity located at 853, and 937  cm −1 were significantly lower for cancerous tissues (p<0.05 ), while the band intensity located at 1094, 1209, 1268, and 1579  cm −1 were significantly higher (p<0.05 ). The band intensity located at 1290 to 1340, and 1660  cm −1 were also higher for cancerous tissues; but the differences were not statistically significant (p<0.05 ). Principal component analysis (PCA) and linear discriminate analysis (LDA) were employed to generate diagnostic algorithms for classification of Raman spectra of the two nasopharyngeal tissue types. The PCA-LDA algorithms together with leave-one-out, cross-validation technique yielded diagnostic sensitivity of 92% and specificity of 82%. This work demonstrated that the Raman spectroscopy technique associated with PCA-LDA diagnostic algorithms has potential for improving the diagnosis of nasopharyngeal cancers.

Paper Details

Date Published: 1 February 2013
PDF: 7 pages
J. Biomed. Opt. 18(2) 027003 doi: 10.1117/1.JBO.18.2.027003
Published in: Journal of Biomedical Optics Volume 18, Issue 2
Show Author Affiliations
Yongzeng Li, Fujian Normal Univ. (China)
Jianji Pan, Fujian Medical Univ. (China)
Guannan Chen, Fujian Normal Univ. (China)
Chao Li, Fujian Medical Univ. (China)
Shaojun Lin, Fujian Medical Univ. (China)
Yonghong Shao, Shenzhen Univ. (China)
Shangyuan Feng, Fujian Normal Univ. (China)
Zufang Huang, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)
Haishan Zeng, The BC Cancer Agency Research Ctr. (Canada)
Rong Chen, Fujian Normal Univ. (China)


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