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Journal of Biomedical Optics

Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo
Author(s): Yuri Dancik; Amandine Favre; Chong Jin Loy; Andrei V. Zvyagin; Michael S. Roberts
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Paper Abstract

There is a growing body of literature showing the usefulness of multiphoton tomography (MPT) and fluorescence lifetime imaging for in situ characterization of skin constituents and the ensuing development of noninvasive diagnostic tools against skin diseases. Melanin and pigmentation-associated skin cancers constitute some of the major applications. We show that MPT and fluorescence lifetime imaging can be used to measure changes in cutaneous melanin concentration and that these can be related to the visible skin color. Melanin in the skin of African, Indian, Caucasian, and Asian volunteers is detected on the basis of its emission wavelength and fluorescence lifetimes in solution and in a melanocyte-keratinocyte cell culture. Fluorescence intensity is used to characterize the melanin content and distribution as a function of skin type and depth into the skin (stratum granulosum and stratum basale). The measured fluorescence intensities in given skin types agree with melanin amounts reported by others using biopsies. Our results suggest that spatial distribution of melanin in skin can be studied using MPT and fluorescence lifetime imaging, but further studies are needed to ascertain that the method can resolve melanin amount in smaller depth intervals.

Paper Details

Date Published: 14 February 2013
PDF: 14 pages
J. Biomed. Opt. 18(2) 026022 doi: 10.1117/1.JBO.18.2.026022
Published in: Journal of Biomedical Optics Volume 18, Issue 2
Show Author Affiliations
Yuri Dancik, The Univ. of Queensland School of Medicine (Australia)
Amandine Favre, The Univ. of Queensland School of Medicine (Australia)
Chong Jin Loy, Johnson & Johnson Asia Pacific (Singapore)
Andrei V. Zvyagin, Macquarie Univ. (Australia)
Michael S. Roberts, The Univ. of Queensland School of Medicine (Australia)

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