Share Email Print

Journal of Biomedical Optics • Open Access

Applying tattoo dye as a third-harmonic generation contrast agent for in vivo optical virtual biopsy of human skin
Author(s): Ming-Rung Tsai; Chen-Yu Lin; Yi-Hua Liao; Chi-Kuang Sun

Paper Abstract

Third-harmonic generation (THG) microscopy has been reported to provide intrinsic contrast in elastic fibers, cytoplasmic membrane, nucleus, actin filaments, lipid bodies, hemoglobin, and melanin in human skin. For advanced molecular imaging, exogenous contrast agents are developed for a higher structural or molecular specificity. We demonstrate the potential of the commonly adopted tattoo dye as a THG contrast agent for in vivo optical biopsy of human skin. Spectroscopy and microscopy experiments were performed on cultured cells with tattoo dyes, in tattooed mouse skin, and in tattooed human skin to demonstrate the THG enhancement effect. Compared with other absorbing dyes or nanoparticles used as exogenous THG contrast agents, tattoo dyes are widely adopted in human skin so that future clinical biocompatibility evaluation is relatively achievable. Combined with the demonstrated THG enhancement effect, tattoo dyes show their promise for future clinical imaging applications.

Paper Details

Date Published: 6 February 2013
PDF: 6 pages
J. Biomed. Opt. 18(2) 026012 doi: 10.1117/1.JBO.18.2.026012
Published in: Journal of Biomedical Optics Volume 18, Issue 2
Show Author Affiliations
Ming-Rung Tsai, National Taiwan Univ. (Taiwan)
Chen-Yu Lin, National Taiwan Univ. (Taiwan)
Yi-Hua Liao, National Taiwan Univ. Hospital (Taiwan)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)

© SPIE. Terms of Use
Back to Top