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Journal of Biomedical Optics • Open Access

Modular video endoscopy for in vivo cross-polarized and vital-dye fluorescence imaging of Barrett’s-associated neoplasia
Author(s): Nadhi Thekkek; Mark C. Pierce; Michelle H. Lee; Alexandros D. Polydorides; Raja M. Flores; Sharmila Anandasabapathy; Rebecca R. Richards-Kortum

Paper Abstract

A modular video endoscope is developed and tested to allow imaging in different modalities. This system incorporates white light imaging (WLI), cross-polarized imaging (CPI), and vital-dye fluorescence imaging (VFI), using interchangeable filter modules. CPI and VFI are novel endoscopic modalities that probe mucosal features associated with Barrett’s neoplasia. CPI enhances vasculature, while VFI enhances glandular architecture. In this pilot study, we demonstrate the integration of these modalities by imaging areas of Barrett’s metaplasia and neoplasia in an esophagectomy specimen. We verify that those key image features are also observed during an in vivo surveillance procedure. CPI images demonstrate improved visualization of branching blood vessels associated with neoplasia. VFI images show glandular architecture with increased glandular effacement associated with neoplasia. Results suggests that important pathologic features seen in CPI and VFI are not visible during standard endoscopic white light imaging, and thus the modalities may be useful in future in vivo studies for discriminating neoplasia from Barrett’s metaplasia. We further demonstrate that the integrated WLI/CPI/VFI endoscope is compatible with complementary high-resolution endomicroscopy techniques such as the high-resolution microendoscope, potentially enabling two-step (“red-flag” widefield plus confirmatory high-resolution imaging) protocols to be enhanced.

Paper Details

Date Published: 1 February 2013
PDF: 9 pages
J. Biomed. Opt. 18(2) 026007 doi: 10.1117/1.JBO.18.2.026007
Published in: Journal of Biomedical Optics Volume 18, Issue 2
Show Author Affiliations
Nadhi Thekkek, Rice Univ. (United States)
Mark C. Pierce, Rutgers, The State Univ. of New Jersey (United States)
Michelle H. Lee, The Mount Sinai Medical Ctr. (United States)
Alexandros D. Polydorides, The Mount Sinai Medical Ctr. (United States)
Raja M. Flores, The Mount Sinai Medical Ctr. (United States)
Sharmila Anandasabapathy, The Mount Sinai Medical Ctr. (United States)
Rebecca R. Richards-Kortum, Rice Univ. (United States)

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