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Journal of Biomedical Optics • Open Access

In vivo dynamic process imaging using real-time optical-resolution photoacoustic microscopy
Author(s): Wei Shi; Peng Shao; Parsin Hajireza; Alexander Forbrich; Roger J. Zemp

Paper Abstract

The authors demonstrate in vivo dynamic process imaging using a label-free real-time optical-resolution photoacoustic microscope (OR-PAM). This reflection-mode system takes advantage of a 532-nm fiber laser source with a high pulse repetition rate of up to 600 kHz combined with a fast-scanning mirror system. Microvasculature in SCID mouse ears is imaged at near real-time (0.5 fps) for a 1×1  mm 2 field of view (FOV) with micron-scale lateral resolution. We also demonstrate imaging of cardiac-induced microhemodynamics in murine microvasculature at real-time frame-rates (30 fps) over a 250×250  μm 2 FOV using real-time C-scan OR-PAM with ability to provide sustained imaging with near real-time feedback for focusing and positioning.

Paper Details

Date Published: 1 February 2013
PDF: 6 pages
J. Biomed. Opt. 18(2) 026001 doi: 10.1117/1.JBO.18.2.026001
Published in: Journal of Biomedical Optics Volume 18, Issue 2
Show Author Affiliations
Wei Shi, Univ. of Alberta (Canada)
Peng Shao, Univ. of Alberta (Canada)
Parsin Hajireza, Univ. of Alberta (Canada)
Alexander Forbrich, Univ. of Alberta (Canada)
Roger J. Zemp, Univ. of Alberta (Canada)

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