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Journal of Biomedical Optics • Open Access

Discrimination of dimethyl sulphoxide diffusion coefficient in the process of optical clearing by confocal micro-Raman spectroscopy
Author(s): Ping Liu; Yanyue Huang; Zhouyi Guo; Jianping Wang; Zhengfei Zhuang; Songhao Liu

Paper Abstract

Confocal micro-Raman spectroscopy is employed to study the diffusion process of dimethyl sulfoxide (DMSO) in porcine skin optical clearing. The variation of DMSO concentration with time at different depths of the skin was obtained and then the DMSO diffusion coefficient with the passive diffusion model was calculated. Results show that it has a significant difference at different depths of the skin. Also, the DMSO concentration with the depth at different times was obtained and the same method was used to find the change law of the DMSO diffusion coefficient. Results indicate that it also changes with the treatment time. The experimental results are consistent with the theoretical model in a previous study. The current results demonstrate that Raman spectroscopy has the ability to quantitatively monitor the process of optical clearing.

Paper Details

Date Published: 15 February 2013
PDF: 4 pages
J. Biomed. Opt. 18(2) 020507 doi: 10.1117/1.JBO.18.2.020507
Published in: Journal of Biomedical Optics Volume 18, Issue 2
Show Author Affiliations
Ping Liu, South China Normal Univ. (China)
Yanyue Huang, South China Normal Univ. (China)
Zhouyi Guo, South China Normal Univ. (China)
Jianping Wang, Guangdong Sensing Optics Science & Technology Co., Ltd. (China)
Zhengfei Zhuang, South China Normal Univ. (China)
Songhao Liu, South China Normal Univ. (China)

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