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Journal of Biomedical Optics • Open Access

Ultrasound modulated imaging of luminescence generated within a scattering medium
Author(s): Nam Trung Huynh; Barrie R. Hayes-Gill; Fan Zhang; Stephen P. Morgan

Paper Abstract

Ultrasound modulated optical tomography modulates scattered light within tissue by deterministically altering the optical properties of the sample with the ultrasonic pressure. This allows the light to be “tagged” and the degradation in spatial resolution associated with light scattering to be reduced. To our knowledge, this is the first demonstration of ultrasound modulated imaging of light generated within a scattering medium without an external light source. The technique has the potential to improve the spatial resolution of chemi- or bioluminescence imaging of tissue. Experimental results show that ultrasound modulated luminescence imaging can resolve two chemiluminescent objects separated by 5 mm at a 7 mm depth within a tissue phantom with a scattering coefficient of 30  cm −1 . The lateral resolution is estimated to be 3 mm. Monte Carlo simulations indicate that, with the current system signal to noise ratio, it is feasible to apply the approach to bioluminescence imaging when the concentration of bacteria in the animal organ is above 3.4×10 5L .

Paper Details

Date Published: 5 February 2013
PDF: 4 pages
J. Biomed. Opt. 18(2) 020505 doi: 10.1117/1.JBO.18.2.020505
Published in: Journal of Biomedical Optics Volume 18, Issue 2
Show Author Affiliations
Nam Trung Huynh, The Univ. of Nottingham (United Kingdom)
Barrie R. Hayes-Gill, The Univ. of Nottingham (United Kingdom)
Fan Zhang, The Univ. of Nottingham (United Kingdom)
Stephen P. Morgan, The Univ. of Nottingham (United Kingdom)

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