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Journal of Biomedical Optics • Open Access

Effects of phototherapy on cartilage structure and inflammatory markers in an experimental model of osteoarthritis
Author(s): Poliani Oliveira; Anderson Amaro Santos; Tamara Rodrigues; Carla Roberta Tim; Karina Zambone Pinto; Angela Maria Paiva Magri; Kelly Rossetti Fernandes; Stela M. Mattiello; Nivaldo Antonio Parizotto; Fernanda Freitas Anibal; Ana Claudia Muniz Renno

Paper Abstract

The aim of this study was to evaluate the effects of laser phototherapy on the degenerative modifications on the articular cartilage after the anterior cruciate ligament transection (ACLT) in the knee of rats. Eighty male rats (Wistar) were distributed into four groups: intact control group (IG), injured control group (CG), injured laser treated group at 10  J/cm 2 (L10), and injured laser treated group at 50  J/cm 2 (L50). Animals were distributed into two subgroups, sacrificed in 5 and 8 weeks postsurgery. The ACLT was used to induce knee osteoarthritis in rats. After 2 weeks postsurgery, laser phototherapy initiated and it was performed for 15 and 30 sessions. The histological findings revealed that laser irradiation, especially at 10  J/cm 2 , modulated the progression of the degenerative process, showing a better cartilage structure and lower number of condrocytes compared to the other groups. Laser phototherapy was not able to decrease the degenerative process measured by Mankin score and prevent the increase of cartilage thickness related to the degenerative process. Moreover, it did not have any effect in the biomodulation of the expression of markers IL1β , tumor necrosis factor-α , and metalloprotein-13. Furthermore, laser irradiated animals, at 50  J/cm 2 showed a lower amount of collagen type 1.

Paper Details

Date Published: 16 December 2013
PDF: 11 pages
J. Biomed. Opt. 18(12) 128004 doi: 10.1117/1.JBO.18.12.128004
Published in: Journal of Biomedical Optics Volume 18, Issue 12
Show Author Affiliations
Poliani Oliveira, Univ. Federal de São Paulo (Brazil)
Anderson Amaro Santos, Univ. Federal de São Paulo (Brazil)
Tamara Rodrigues, Univ. Federal de São Paulo (Brazil)
Carla Roberta Tim, Univ. Federal de São Paulo (Brazil)
Karina Zambone Pinto, Univ. Federal de São Paulo (Brazil)
Angela Maria Paiva Magri, Univ. Federal de São Paulo (Brazil)
Kelly Rossetti Fernandes, Univ. Federal de São Paulo (Brazil)
Stela M. Mattiello, Univ. Federal de São Paulo (Brazil)
Nivaldo Antonio Parizotto, Univ. Federal de São Paulo (Brazil)
Fernanda Freitas Anibal, Univ. Federal de São Paulo (Brazil)
Ana Claudia Muniz Renno, Univ. Federal de São Paulo (Brazil)

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