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Journal of Biomedical Optics

Effect of 940 nm low-level laser therapy on osteogenesis in vitro
Author(s): Mohammed M. Jawad; Adam Husein; Ahmad Azlina; Mohammad Khursheed Alam; Rozita Hassan; Rumaizi Shaari
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Paper Abstract

Bone regeneration is essential in medical treatment, such as in surgical bone healing and orthodontics. The aim of this study is to examine the effect of different powers of 940 nm diode low-level laser treatment (LLLT) on osteoblast cells during their proliferation and differentiation stages. A human fetal osteoblast cell line was cultured and treated with LLLT. The cells were divided into experimental groups according to the power delivered and periods of exposure per day for each laser power. The (3-(4,5-dimethylthiazol-2yl)-2,5 diphenyl tetrazolium bromide) (MTT) assay was used to determine cell proliferation. Both alkaline phosphatase and osteocalcin activity assays were assessed for cell differentiation. All treatment groups showed a significant increase in cell proliferation and differentiation compared to the control group. Regarding the exposure time, the subgroups treated with the LLLT for 6 min showed higher proliferation and differentiation rates for the powers delivered, the 300-mW LLLT group significantly increased the amount of cell proliferation. By contrast, the 100 and 200 mW groups showed significantly greater amounts of cell differentiation. These results suggest that the use of LLLT may play an important role in stimulating osteoblast cells for improved bone formation.

Paper Details

Date Published: 10 December 2013
PDF: 7 pages
J. Biomed. Opt. 18(12) 128001 doi: 10.1117/1.JBO.18.12.128001
Published in: Journal of Biomedical Optics Volume 18, Issue 12
Show Author Affiliations
Mohammed M. Jawad, Univ. Sains Malaysia (Malaysia)
Adam Husein, Univ. Sains Malaysia (Malaysia)
Ahmad Azlina, Univ. Sains Malaysia (Malaysia)
Mohammad Khursheed Alam, Univ. Sains Malaysia (Malaysia)
Rozita Hassan, Univ. Sains Malaysia (Malaysia)
Rumaizi Shaari, Univ. Sains Malaysia (Malaysia)

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