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Journal of Biomedical Optics • Open Access

Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy
Author(s): Zufang Huang; Yan Sun; Jing Wang; Shengrong Du; Yongzeng Li; Juqiang Lin; Shangyuan Feng; Jinping Lei; Hongxin Lin; Rong Chen; Haishan Zeng

Paper Abstract

In this study, a rapid and simple method which combines drop coating deposition and Raman spectroscopy (DCDR) was developed to characterize the dry embryo culture media (ECM) droplet. We demonstrated that Raman spectra obtained from the droplet edge presented useful and characteristic signatures for protein and amino acids assessment. Using a different analytical method, scanning electron microscopy coupled with energy dispersive X-ray analysis, we further confirmed that Na, K, and Cl were mainly detected in the central area of the dry ECM droplet while sulphur, an indicative of the presence of macromolecules such as proteins, was mainly found at the periphery of the droplet. In addition, to reduce sample preparation time, different temperatures for drying the droplets were tested. The results showed that drying temperature at 50°C can effectively reduce the sample preparation time to 6 min (as compared to 50 min for drying at room temperature, ∼25°C ) without inducing thermal damage to the proteins. This work demonstrated that DCDR has potential for rapid and reliable metabolomic profiling of ECM in clinical applications.

Paper Details

Date Published: 16 December 2013
PDF: 6 pages
J. Biomed. Opt. 18(12) 127003 doi: 10.1117/1.JBO.18.12.127003
Published in: Journal of Biomedical Optics Volume 18, Issue 12
Show Author Affiliations
Zufang Huang, Fujian Normal Univ. (China)
Yan Sun, Fujian Maternal and Child Health Hospital (China)
Jing Wang, Fujian Normal Univ. (China)
Shengrong Du, Fujian Maternal and Child Health Hospital (China)
Yongzeng Li, Fujian Normal Univ. (China)
Juqiang Lin, Fujian Normal Univ. (China)
Shangyuan Feng, Fujian Normal Univ. (China)
Jinping Lei, Fujian Normal Univ. (China)
Hongxin Lin, Fujian Normal Univ. (China)
Rong Chen, Fujian Normal Univ. (China)
Haishan Zeng, The BC Cancer Agency Research Ctr. (Canada)

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