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Journal of Biomedical Optics

mPlum-IFP 1.4 fluorescent fusion protein may display Förster resonance energy transfer associated properties that can be used for near-infrared based reporter gene imaging
Author(s): Liang-Ting Lin; Bo-Sheng Wang; Jyh-Cheng Chen; Chi-Hsien Liu; Chien Chou; Shu-Jun Chiu; Wen-Yi Chang; Ren-Shyan Liu; C. Allen Chang; Yi-Jang Lee
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Paper Abstract

Bacteriophytochrome infrared fluorescent protein (IFP) has a long emission wavelength that is appropriate for detecting pathophysiological effects via near-infrared (NIR) based imaging. However, the brightness and photostability of IFP are suboptimal, although an exogenous supply of biliverdin (BV) IXα is able to enhance these properties. In this study, we fused a far red mPlum fluorescent protein to IFP 1.4 via a linker deoxyribonucleic acid (DNA) sequence encoding eight amino acids. The brightness of mPlum-IFP 1.4 fusion protein at the IFP emission channel was comparable to that of native IFP 1.4 protein when fusion protein and IFP 1.4 were excited by 543 and 633 nm using confocal microscopy, respectively. Visualization of IFP 1.4 fluorescence by excitation of mPlum in mPlum-IFP 1.4 fusion protein is likely to be associated with Förster resonance energy transfer (FRET). The FRET phenomenon was also predicted by acceptor photobleaching using confocal microscopy. Furthermore, the expression of mPlum-IFP 1.4 fusion protein could be detected in cell culture and in xenograft tumors in the absence of BV using in vivo imaging system, although the BV was still essential for detecting native IFP 1.4. Therefore, this innovativefluorescent fusion protein would be useful for NIR-based imaging in vitro and in vivo.

Paper Details

Date Published: 16 December 2013
PDF: 10 pages
J. Biomed. Opt. 18(12) 126013 doi: 10.1117/1.JBO.18.12.126013
Published in: Journal of Biomedical Optics Volume 18, Issue 12
Show Author Affiliations
Liang-Ting Lin, National Yang-Ming Univ. (Taiwan)
Bo-Sheng Wang, National Yang-Ming Univ. (Taiwan)
Jyh-Cheng Chen, National Yang-Ming Univ. (Taiwan)
Chi-Hsien Liu, Chang Gung Univ. (Taiwan)
Chien Chou, Chang Gung Univ. (Taiwan)
Shu-Jun Chiu, Tzu Chi Univ. (Taiwan)
Wen-Yi Chang, National Yang-Ming Univ. (Taiwan)
Ren-Shyan Liu, National Yang-Ming Univ. (Taiwan)
C. Allen Chang, National Yang-Ming Univ. (Taiwan)
Yi-Jang Lee, National Yang-Ming Univ. (Taiwan)

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