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Journal of Biomedical Optics • Open Access

Vasodilation by in vivo activation of astrocyte endfeet via two-photon calcium uncaging as a strategy to prevent brain ischemia
Author(s): Yuanxin Chen; James J. Mancuso; Zhen Zhao; Xuping Li; Jie Cheng; Gustavo Roman; Stephen T. Wong

Paper Abstract

Decreased cerebral blood flow causes brain ischemia and plays an important role in the pathophysiology of many neurodegenerative diseases, including Alzheimer’s disease and vascular dementia. In this study, we photomodulated astrocytes in the live animal by a combination of two-photon calcium uncaging in the astrocyte endfoot and in vivo imaging of neurovasculature and astrocytes by intravital two-photon microscopy after labeling with cell type specific fluorescent dyes. Our study demonstrates that photomodulation at the endfoot of a single astrocyte led to a 25% increase in the diameter of a neighboring arteriole, which is a crucial factor regulating cerebral microcirculation in downstream capillaries. Two-photon uncaging in the astrocyte soma or endfoot near veins does not show the same effect on microcirculation. These experimental results suggest that infrared photomodulation on astrocyte endfeet may be a strategy to increase cerebral local microcirculation and thus prevent brain ischemia.

Paper Details

Date Published: 16 December 2013
PDF: 6 pages
J. Biomed. Opt. 18(12) 126012 doi: 10.1117/1.JBO.18.12.126012
Published in: Journal of Biomedical Optics Volume 18, Issue 12
Show Author Affiliations
Yuanxin Chen, The Methodist Hospital Research Institute (United States)
James J. Mancuso, The Methodist Hospital Research Institute (United States)
Zhen Zhao, The Methodist Hospital Research Institute (United States)
Xuping Li, The Methodist Hospital Research Institute (United States)
Jie Cheng, The Methodist Hospital Research Institute (United States)
Gustavo Roman, The Methodist Hospital Research Institute (United States)
Stephen T. Wong, The Methodist Hospital Research Institute (United States)


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