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Journal of Biomedical Optics • Open Access

Nanosecond ratio imaging of redox states in tumor cell spheroids using light sheet-based fluorescence microscopy
Author(s): Sarah Schickinger; Thomas Bruns; Rainer Wittig; Petra Weber; Michael Wagner; Herbert Schneckenburger

Paper Abstract

A new concept of three-dimensional imaging of tumor cell spheroids by light sheet-based fluorescence microscopy and nanosecond ratio imaging is described. Due to its low light dose and alternative excitation by two laser wavelengths (391 and 470 nm), this method maintains cell viability and permits recording of real-time kinetics. A genetically encoded sensor permits measurement of the redox state of glutathione and visualization of the impact of oxygen radicals. The pharmaceutically relevant system is tested upon addition of an oxidizing agent (H2O2 ), as well as upon addition of the apoptosis-inducing agent staurosporine.

Paper Details

Date Published: 16 December 2013
PDF: 6 pages
J. Biomed. Opt. 18(12) 126007 doi: 10.1117/1.JBO.18.12.126007
Published in: Journal of Biomedical Optics Volume 18, Issue 12
Show Author Affiliations
Sarah Schickinger, Hochschule Aalen (Germany)
Thomas Bruns, Hochschule Aalen (Germany)
Rainer Wittig, Institut für Lasertechnologien in der Medizin und Messtechnik (Germany)
Petra Weber, Hochschule Aalen (Germany)
Michael Wagner, Hochschule Aalen (Germany)
Herbert Schneckenburger, Hochschule Aalen (Germany)


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