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Journal of Biomedical Optics • Open Access

Experimental and analytical comparative study of optical coefficient of fresh and frozen rat tissues
Author(s): Mohammed Mesradi; Aurelie Genoux; Vesna Cuplov; Darine Abi-Haidar; Sebastien Jan; Irene Buvat; Frederic Pain

Paper Abstract

Optical properties of fresh and frozen tissues of rat heart, kidney, brain, liver, and muscle were measured in the 450- to 700-nm range. The total reflectance and transmittance were measured using a well-calibrated integral sphere set-up. Absorption coefficient μa and reduced scattering coefficient μ′s were derived from the experimental measurements using the inverse adding doubling technique. The influence of cryogenic processing on optical properties was studied. Interindividual and intraindividual variations were assessed. These new data aim at filling the lack of validated optical properties in the visible range especially in the blue-green region of particular interest for fluorescence and optogenetics preclinical studies. Furthermore, we provide a unique comparison of the optical properties of different organs obtained using the same measurement set-up for fresh and frozen tissues as well as an estimate of the intraindividual and interindividual variability.

Paper Details

Date Published: 18 November 2013
PDF: 9 pages
J. Biomed. Opt. 18(11) 117010 doi: 10.1117/1.JBO.18.11.117010
Published in: Journal of Biomedical Optics Volume 18, Issue 11
Show Author Affiliations
Mohammed Mesradi, Univ. Paris-Sud 11 (France)
Aurelie Genoux, Univ. Paris-Sud 11 (France)
Vesna Cuplov, Service Hospitalier Frédéric Joliot (France)
Darine Abi-Haidar, Univ. Paris-Sud 11 (France)
Sebastien Jan, Service Hospitalier Frédéric Joliot (France)
Irene Buvat, Univ. Paris-Sud 11 (France)
Frederic Pain, Univ. Paris-Sud 11 (France)

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