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Journal of Biomedical Optics • Open Access

Application of Raman spectroscopy for visualizing biochemical changes during peripheral nerve injury in vitro and in vivo
Author(s): Shinsuke Morisaki; Chikashi Ota; Ken-ichi Matsuda; Natsuko Kaku; Hiroyoshi Fujiwara; Ryo Oda; Hidenobu Ishibashi; Toshikazu Kubo; Mitsuhiro Kawata

Paper Abstract

Raman spectroscopy can be used for analysis of objects by detecting the vibrational spectrum using label-free methods. This imaging method was applied to analysis of peripheral nerve regeneration by examining the sciatic nerve in vitro and in vivo. Raman spectra of intact nerve tissue had three particularly important peaks in the range 28003000  cm1 . Spectra of injured sciatic nerves showed significant changes in the ratio of these peaks. Analysis of cellular spectra suggested that the spectrum for sciatic nerve tissue reflects the axon and myelin components of this tissue. Immunohistochemical analysis showed that the number of axons and the myelinated area were reduced at 7 days after injury and then increased by 28 days. The relative change in the axon to myelin ratio showed a similar initial increase, followed by a decrease at 28 days after injury. These changes correlated with the band intensity ratio and the changes in distribution of axon and myelin in Raman spectral analysis. Thus, our results suggest that label-free biochemical imaging with Raman spectroscopy can be used to detect turnover of axon and myelin in peripheral nerve regeneration.

Paper Details

Date Published: 26 November 2013
PDF: 9 pages
J. Biomed. Opt. 18(11) 116011 doi: 10.1117/1.JBO.18.11.116011
Published in: Journal of Biomedical Optics Volume 18, Issue 11
Show Author Affiliations
Shinsuke Morisaki, Kyoto Prefectural Univ. of Medicine (Japan)
Chikashi Ota, HORIBA, Ltd. (Japan)
Ken-ichi Matsuda, Kyoto Prefectural Univ. of Medicine (Japan)
Natsuko Kaku, HORIBA, Ltd. (Japan)
Hiroyoshi Fujiwara, Kyoto Prefectural Univ. of Medicine (Japan)
Ryo Oda, Kyoto Prefectural Univ. of Medicine (Japan)
Hidenobu Ishibashi, Kyoto Prefectural Univ. of Medicine (Japan)
Toshikazu Kubo, Kyoto Prefectural Univ. of Medicine (Japan)
Mitsuhiro Kawata, Kyoto Prefectural Univ. of Medicine (Japan)


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