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Journal of Biomedical Optics

Wide-field interferometric phase microscopy with molecular specificity using plasmonic nanoparticles
Author(s): Nir A. Turko; Anna Peled; Natan T. Shaked
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Paper Abstract

We present a method for adding molecular specificity to wide-field interferometric phase microscopy (IPM) by recording the phase signatures of gold nanoparticles (AuNPs) labeling targets of interest in biological cells. The AuNPs are excited by time-modulated light at a wavelength corresponding to their absorption spectral peak, evoking a photothermal (PT) effect due to their plasmonic resonance. This effect induces a local temperature rise, resulting in local refractive index and phase changes that can be detected optically. Using a wide-field interferometric phase microscope, we acquired an image sequence of the AuNP sample phase profile without requiring lateral scanning, and analyzed the time-dependent profile of the entire field of view using a Fourier analysis, creating a map of the locations of AuNPs in the sample. The system can image a wide-field PT phase signal from a cluster containing down to 16 isolated AuNPs. AuNPs are then conjugated to epidermal growth factor receptor (EGFR) antibodies and inserted to an EGFR-overexpressing cancer cell culture, which is imaged using IPM and verified by confocal microscopy. To the best of our knowledge, this is the first time wide-field interferometric PT imaging is performed at the subcellular level without the need for total internal reflection effects or scanning.

Paper Details

Date Published: 27 September 2013
PDF: 9 pages
J. Biomed. Opt. 18(11) 111414 doi: 10.1117/1.JBO.18.11.111414
Published in: Journal of Biomedical Optics Volume 18, Issue 11
Show Author Affiliations
Nir A. Turko, Tel Aviv Univ. (Israel)
Anna Peled, Tel Aviv Univ. (Israel)
Natan T. Shaked, Tel Aviv Univ. (Israel)


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