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Journal of Biomedical Optics • Open Access

Localized cell stiffness measurement using axial movement of an optically trapped microparticle
Author(s): Mary-Clare C. Dy; Shigehiko Kanaya; Tadao Sugiura

Paper Abstract

A simple optical tweezers design is proposed to manipulate particles in the axial direction and estimate particle position with nanometer sensitivity. Balb3T3 cell is probed using two different-sized particles, and the localized cell stiffness is evaluated using Hertz model. A series of experiments are performed to obtain the necessary parameters for the cell stiffness computation: particle displacement, trapping stiffness, force exertion, and cell deformation. The computed cell stiffness measurements are 17 and 40 Pa using 4 μm- and 2 μm-sized particles, respectively. Results suggest that the proposed optical tweezers scheme can measure the stiffness of a particular cell locale using Hertz model, offering insights about how cells respond to outside mechanical stimulus.

Paper Details

Date Published: 9 August 2013
PDF: 5 pages
J. Biomed. Opt. 18(11) 111411 doi: 10.1117/1.JBO.18.11.111411
Published in: Journal of Biomedical Optics Volume 18, Issue 11
Show Author Affiliations
Mary-Clare C. Dy, Nara Institute of Science and Technology (Japan)
Shigehiko Kanaya, Nara Institute of Science and Technology (Japan)
Tadao Sugiura, Nara Institute of Science and Technology (Japan)

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