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Journal of Biomedical Optics

On-chip optical stimulation and electrical recording from cells
Author(s): Alexey Yakushenko; Zheng Gong; Vanessa Maybeck; Boris Hofmann; Erdan Gu; Martin D. Dawson; Andreas Offenhaeusser; Bernhard Wolfrum
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Paper Abstract

We present an optoelectrical device capable of in vitro optical stimulation and electrophysiological recording. The device consists of an array of micropixellated InGaN light-emitting diodes coupled to a custom-made ultrathin planar microelectrode array. Cells can be cultured directly on the chip for short- and long-term electrophysiological experiments. To show the functionality of the device, we transfected a cardiomyocyte-like cell line (HL-1) with a light-sensitive protein channelrhodopsin. We monitored action potentials of individual, spontaneously beating, HL-1 cells growing on the chip by extracellular electrical recordings. On-chip optical stimulation was demonstrated by triggering network activity in a confluent HL-1 cell culture and visualized by calcium imaging. We see the potential of our system for electrophysiological experiments with optogenetically modified cells. Optical stimulation can be performed directly on the chip without additional optical components or external light sources.

Paper Details

Date Published: 20 June 2013
PDF: 8 pages
J. Biomed. Opt. 18(11) 111402 doi: 10.1117/1.JBO.18.11.111402
Published in: Journal of Biomedical Optics Volume 18, Issue 11
Show Author Affiliations
Alexey Yakushenko, Forschungszentrum Jülich GmbH (Germany)
Zheng Gong, Univ. of Strathclyde (United Kingdom)
Vanessa Maybeck, Forschungszentrum Jülich GmbH (Germany)
Boris Hofmann, Forschungszentrum Jülich GmbH (Germany)
Erdan Gu, Univ. of Strathclyde (United Kingdom)
Martin D. Dawson, Univ. of Strathclyde (United Kingdom)
Andreas Offenhaeusser, Forschungszentrum Jülich GmbH (Germany)
Bernhard Wolfrum, Forschungszentrum Jülich GmbH (Germany)

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