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Journal of Biomedical Optics • Open Access

Dynamic near-infrared imaging reveals transient phototropic change in retinal rod photoreceptors
Author(s): Rongwen Lu; Alexander M. Levy; Qiuxiang Zhang; Steven J. Pittler; Xincheng Yao

Paper Abstract

Stiles–Crawford effect (SCE) is exclusively observed in cone photoreceptors, but why the SCE is absent in rod photoreceptors is still a mystery. In this study, we employed dynamic near infrared light imaging to monitor photoreceptor kinetics in freshly isolated frog and mouse retinas stimulated by oblique visible light flashes. It was observed that retinal rods could rapidly (onset: ∼10  ms for frog and 5 ms for mouse; time-to-peak: ∼200  ms for frog and 30 ms for mouse) shift toward the direction of the visible light, which might quickly compensate for the loss of luminous efficiency due to oblique illumination. In contrast, such directional movement was negligible in retinal cones. Moreover, transient rod phototropism could contribute to characteristic intrinsic optical signal (IOS). We anticipate that further study of the transient rod phototropism may not only provide insight into better understanding of the nature of vision but also promise an IOS biomarker for functional mapping of rod physiology at high resolution.

Paper Details

Date Published: 28 October 2013
PDF: 7 pages
J. Biomed. Opt. 18(10) 106013 doi: 10.1117/1.JBO.18.10.106013
Published in: Journal of Biomedical Optics Volume 18, Issue 10
Show Author Affiliations
Rongwen Lu, The Univ. of Alabama at Birmingham (United States)
Alexander M. Levy, The Univ. of Alabama at Birmingham (United States)
Qiuxiang Zhang, The Univ. of Alabama at Birmingham (United States)
Steven J. Pittler, The Univ. of Alabama at Birmingham (United States)
Xincheng Yao, The Univ. of Alabama at Birmingham (United States)

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