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Journal of Biomedical Optics • Open Access

Multimodal tissue imaging: using coregistered optical tomography data to estimate tissue autofluorescence intensity change due to scattering and absorption by neoplastic epithelial cells
Author(s): Hamid Pahlevaninezhad; Ivana Cecic; Anthony M. Lee; Alastair H. Kyle; Stephen Lam; Calum MacAulay; Pierre M. Lane

Paper Abstract

Autofluorescence (AF) imaging provides valuable information about the structural and chemical states of tissue that can be used for early cancer detection. Optical scattering and absorption of excitation and emission light by the epithelium can significantly affect observed tissue AF intensity. Determining the effect of epithelial attenuation on the AF intensity could lead to a more accurate interpretation of AF intensity. We propose to use optical coherence tomography coregistered with AF imaging to characterize the AF attenuation due to the epithelium. We present imaging results from three vital tissue models, each consisting of a three-dimensional tissue culture grown from one of three epithelial cell lines (HCT116, OVCAR8, and MCF7) and immobilized on a fluorescence substrate. The AF loss profiles in the tissue layer show two different regimes, each approximately linearly decreasing with thickness. For thin cell cultures (<300  μm ), the AF signal changes as AF(t)/AF(0)=1−1.3[i]t ([b]t is the thickness in millimeter). For thick cell cultures (<400  μm ), the AF loss profiles have different intercepts but similar slopes. The data presented here can be used to estimate AF loss due to a change in the epithelial layer thickness and potentially to reduce AF bronchoscopy false positives due to inflammation and non-neoplastic epithelial thickening.

Paper Details

Date Published: 9 October 2013
PDF: 9 pages
J. Biomed. Opt. 18(10) 106007 doi: 10.1117/1.JBO.18.10.106007
Published in: Journal of Biomedical Optics Volume 18, Issue 10
Show Author Affiliations
Hamid Pahlevaninezhad, The BC Cancer Agency Research Ctr. (Canada)
Ivana Cecic, The BC Cancer Agency Research Ctr. (Canada)
Anthony M. Lee, The BC Cancer Agency Research Ctr. (Canada)
Alastair H. Kyle, The BC Cancer Agency Research Ctr. (Canada)
Stephen Lam, The BC Cancer Agency Research Ctr. (Canada)
Calum MacAulay, The BC Cancer Agency Research Ctr. (Canada)
Pierre M. Lane, The BC Cancer Agency Research Ctr. (Canada)

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