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Journal of Biomedical Optics

High-resolution optoacoustic mesoscopy with a 24 MHz multidetector translate-rotate scanner
Author(s): Jérôme Gateau; Andrei Chekkoury; Vasilis Ntziachristos
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Paper Abstract

Optoacoustic (photoacoustic) mesoscopy aims at high-resolution optical imaging of anatomical, functional, and cellular parameters at depths that go well beyond those of optical-resolution optical or optoacoustic microscopy i.e., reaching several millimeters in depth. The approach utilizes tomography to achieve ultrasonic-diffraction resolution and operates at high-ultrasound frequencies (20 to 200 MHz) induced by few-nanosecond laser pulse excitation of tissues. We investigated here the performance of optoacoustic mesoscopy implemented at 24 MHz center frequency and its ability to resolve optical absorption contrast in the mouse kidney ex vivo. The developed system achieved better than 30 μm in-plane resolution and 110 μm elevation resolution over a cylindrical volume of 9-mm diameter and 9-mm height. This unprecedented combination of resolution and depth was achieved by implementing a translate-rotate detection geometry and by tomographic reconstruction. The approach yielded images of optically absorbing structures with a level of detail never-before visualized in an intact mouse kidney and allows insights into their unperturbed architecture. We discuss the ability to offer multispectral acquisitions and enable in vivo imaging.

Paper Details

Date Published: 4 October 2013
PDF: 11 pages
J. Biomed. Opt. 18(10) 106005 doi: 10.1117/1.JBO.18.10.106005
Published in: Journal of Biomedical Optics Volume 18, Issue 10
Show Author Affiliations
Jérôme Gateau, Helmholtz Zentrum München GmbH (Germany)
Andrei Chekkoury, Helmholtz Zentrum München GmbH (Germany)
Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany)

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