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Journal of Biomedical Optics

Photoacoustic recovery after photothermal bleaching in living cells
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Paper Abstract

We present an innovative method, photoacoustic recovery after photothermal bleaching (PRAP), for studying particle dynamics at micron scale via photoacoustic imaging. As an intuitive way to visualize and quantify dynamic processes, PRAP is demonstrated first in a simple phantom study and then in a more complex measurement involving live cells. Compared with the conventional fluorescence-based approach, PRAP provides high signal-to-noise ratio (SNR) imaging with minimal bleaching-induced artifacts during the recovery stage, ideal for monitoring the diffusive and kinetic processes inside a cell.

Paper Details

Date Published: 2 October 2013
PDF: 6 pages
J. Biomed. Opt. 18(10) 106004 doi: 10.1117/1.JBO.18.10.106004
Published in: Journal of Biomedical Optics Volume 18, Issue 10
Show Author Affiliations
Chiye Li, Washington Univ. in St. Louis (United States)
Chi Zhang, Washington Univ. in St. Louis (United States)
Liang Gao, Washington Univ. in St. Louis (United States)
Alejandro Garcia-Uribe, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)

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