Share Email Print
cover

Journal of Biomedical Optics

Multispectral imaging in the extended near-infrared window based on endogenous chromophores
Author(s): Qian Cao; Natalia G. Zhegalova; Steven T. Wang; Walter J. Akers; Mikhail Y. Berezin
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

To minimize the problem with scattering in deep tissues while increasing the penetration depth, we explored the feasibility of imaging in the relatively unexplored extended near infrared (exNIR) spectral region at 900 to 1400 nm with endogenous chromophores. This region, also known as the second NIR window, is weakly dominated by absorption from water and lipids and is free from other endogenous chromophores with virtually no autofluorescence. To demonstrate the applicability of the exNIR for bioimaging, we analyzed the optical properties of individual components and biological tissues using an InGaAs spectrophotometer and a multispectral InGaAs scanning imager featuring transmission geometry. Based on the differences in spectral properties of tissues, we utilized ratiometric approaches to extract spectral characteristics from the acquired three-dimensional “datacube”. The obtained images of an exNIR transmission through a mouse head revealed sufficient details consistent with anatomical structures.

Paper Details

Date Published: 12 August 2013
PDF: 8 pages
J. Biomed. Opt. 18(10) 101318 doi: 10.1117/1.JBO.18.10.101318
Published in: Journal of Biomedical Optics Volume 18, Issue 10
Show Author Affiliations
Qian Cao, Washington Univ. School of Medicine in St. Louis (United States)
Natalia G. Zhegalova, Washington Univ. School of Medicine in St. Louis (United States)
Steven T. Wang, Washington Univ. School of Medicine in St. Louis (United States)
Walter J. Akers, Washington Univ. School of Medicine in St. Louis (United States)
Mikhail Y. Berezin, Washington Univ. School of Medicine in St. Louis (United States)


© SPIE. Terms of Use
Back to Top