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Journal of Biomedical Optics

Speckle-based configuration for simultaneous in vitro inspection of mechanical contractions of cardiac myocyte cells
Author(s): Mark Golberg; Dror Fixler; Asher Shainberg; Sharon Zlochiver; Vicente Micó; Javier Garcia; Yevgeny Beiderman; Zeev Zalevsky
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Paper Abstract

An optical lensless configuration for a remote noncontact measuring of mechanical contractions of a vast number of cardiac myocytes is proposed. All the myocytes were taken from rats, and the measurements were done in an in vitro mode. The optical method is based on temporal analysis of secondary reflected speckle patterns generated in lensless microscope configuration. The processing involves analyzing the movement and the change in the statistics of the secondary speckle patterns that are created on top of the cell culture when it is illuminated by a spot of laser beam. The main advantage of the proposed system is the ability to measure many cells simultaneously (∼1000 cells) and to extract the statistical data of their movement at once. The presented experimental results also include investigation of the effect of isoproteranol on cell contraction process.

Paper Details

Date Published: 15 July 2013
PDF: 6 pages
J. Biomed. Opt. 18(10) 101310 doi: 10.1117/1.JBO.18.10.101310
Published in: Journal of Biomedical Optics Volume 18, Issue 10
Show Author Affiliations
Mark Golberg, Tel Aviv Univ. (Israel)
Dror Fixler, Bar-Ilan Univ. (Israel)
Asher Shainberg, Bar-Ilan Univ. (Israel)
Sharon Zlochiver, Tel Aviv Univ. (Israel)
Vicente Micó, Univ. de València (Spain)
Javier Garcia, Univ. de València (Spain)
Yevgeny Beiderman, Bar-Ilan Univ. (Israel)
Zeev Zalevsky, Bar-Ilan Univ. (Israel)

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