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Journal of Biomedical Optics

Mesoscopic fluorescence molecular tomography of reporter genes in bioprinted thick tissue
Author(s): Mehmet S. Ozturk; Vivian K. Lee; Lingling Zhao; Guohoa Dai; Xavier Intes
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Paper Abstract

Three-dimensional imaging of thick tissue constructs is one of the main challenges in the field of tissue engineering and regenerative medicine. Optical methods are the most promising as they offer noninvasive, fast, and inexpensive solutions. Herein, we report the use of mesoscopic fluorescence molecular tomography (MFMT) to image function and structure of thick bioprinted tissue hosted in a 3-mm-thick bioreactor. Collagen-based tissue assembled in this study contains two vascular channels formed by green fluorescent protein- and mCherry-expressing cells. Transfected live cell imaging enables us to image function, whereas Flash Red fluorescent bead perfusion into the vascular channel allows us to image structure. The MFMT optical reconstructions are benchmarked with classical microscopy techniques. MFMT and wide-field fluorescence microscopy data match within 92% in area and 84% in location, validating the accuracy of MFMT reconstructions. Our results demonstrate that MFMT is a well-suited imaging modality for fast, longitudinal, functional imaging of thick, and turbid tissue engineering constructs.

Paper Details

Date Published: 3 October 2013
PDF: 4 pages
J. Biomed. Opt. 18(10) 100501 doi: 10.1117/1.JBO.18.10.100501
Published in: Journal of Biomedical Optics Volume 18, Issue 10
Show Author Affiliations
Mehmet S. Ozturk, Rensselaer Polytechnic Institute (United States)
Vivian K. Lee, Rensselaer Polytechnic Institute (United States)
Lingling Zhao, Rensselaer Polytechnic Institute (United States)
Guohoa Dai, Rensselaer Polytechnic Institute (United States)
Xavier Intes, Rensselaer Polytechnic Institute (United States)

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