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Journal of Biomedical Optics

Accurate measurement of volume and shape of resting and activated blood platelets from light scattering
Author(s): Alexander E. Moskalensky; Maxim A. Yurkin; Anastasiya I. Konokhova; Dmitry I. Strokotov; Vyacheslav M. Nekrasov; Andrei V. Chernyshev; Galina A. Tsvetovskaya; Elena D. Chikova; Valeri P. Maltsev
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Paper Abstract

We introduce a novel approach for determination of volume and shape of individual blood platelets modeled as an oblate spheroid from angle-resolved light scattering with flow-cytometric technique. The light-scattering profiles (LSPs) of individual platelets were measured with the scanning flow cytometer and the platelet characteristics were determined from the solution of the inverse light-scattering problem using the precomputed database of theoretical LSPs. We revealed a phenomenon of parameter compensation, which is partly explained in the framework of anomalous diffraction approximation. To overcome this problem, additional a priori information on the platelet refractive index was used. It allowed us to determine the size of each platelet with subdiffraction precision and independent of the particular value of the platelet aspect ratio. The shape (spheroidal aspect ratio) distributions of platelets showed substantial differences between native and activated by 10 μM adenosine diphosphate samples. We expect that the new approach may find use in hematological analyzers for accurate measurement of platelet volume distribution and for determination of the platelet activation efficiency.

Paper Details

Date Published: 3 January 2013
PDF: 5 pages
J. Biomed. Opt. 18(1) 017001 doi: 10.1117/1.JBO.18.1.017001
Published in: Journal of Biomedical Optics Volume 18, Issue 1
Show Author Affiliations
Alexander E. Moskalensky, Institute of Chemical Kinetics and Combustion (Russian Federation)
Maxim A. Yurkin, Institute of Chemical Kinetics and Combustion (Russian Federation)
Anastasiya I. Konokhova, Institute of Chemical Kinetics and Combustion (Russian Federation)
Dmitry I. Strokotov, Institute of Chemical Kinetics and Combustion (Russian Federation)
Vyacheslav M. Nekrasov, Institute of Chemical Kinetics and Combustion (Russian Federation)
Andrei V. Chernyshev, Institute of Chemical Kinetics and Combustion (Russian Federation)
Galina A. Tsvetovskaya, Ctr. for New Medical Technologies (TEMPO) (Russian Federation)
Elena D. Chikova, Novosibirsk State Univ. (Russian Federation)
Valeri P. Maltsev, Institute of Chemical Kinetics and Combustion (Russian Federation)


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