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Journal of Biomedical Optics

Optical imaging of mitochondrial redox state in rodent model of retinitis pigmentosa
Author(s): Sepideh Maleki; Sandeep Gopalakrishnan; Zahra Ghanian; Reyhaneh Sepehr; Heather Schmitt; Janis T. Eells; Mahsa Ranji
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Paper Abstract

Oxidative stress (OS) and mitochondrial dysfunction contribute to photoreceptor cell loss in retinal degenerative disorders. The metabolic state of the retina in a rodent model of retinitis pigmentosa (RP) was investigated using a cryo-fluorescence imaging technique. The mitochondrial metabolic coenzymes nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) are autofluorescent and can be monitored without exogenous labels using optical techniques. The cryo-fluorescence redox imaging technique provides a quantitative assessment of the metabolism. More specifically, the ratio of the fluorescence intensity of these fluorophores (NADH/FAD), the NADH redox ratio (RR), is a marker of the metabolic state of the tissue. The NADH RR and retinal function were examined in an established rodent model of RP, the P23H rat compared to that of nondystrophic Sprague-Dawley (SD) rats. The NADH RR mean values were 1.11±0.03 in the SD normal and 0.841±0.01 in the P23H retina, indicating increased OS in the P23H retina. Electroretinographic data revealed a significant reduction in photoreceptor function in P23H animals compared to SD nozrmal rats. Thus, cryo-fluorescence redox imaging was used as a quantitative marker of OS in eyes from transgenic rats and demonstrated that alterations in the oxidative state of eyes occur during the early stages of RP.

Paper Details

Date Published: 4 January 2013
PDF: 8 pages
J. Biomed. Opt. 18(1) 016004 doi: 10.1117/1.JBO.18.1.016004
Published in: Journal of Biomedical Optics Volume 18, Issue 1
Show Author Affiliations
Sepideh Maleki, Univ. of Wisconsin-Milwaukee (United States)
Sandeep Gopalakrishnan, Univ. of Wisconsin-Milwaukee (United States)
Zahra Ghanian, Univ. of Wisconsin-Milwaukee (United States)
Reyhaneh Sepehr, Univ. of Wisconsin-Milwaukee (United States)
Heather Schmitt, Univ. of Wisconsin-Milwaukee (United States)
Janis T. Eells, Univ. of Wisconsin-Milwaukee (United States)
Mahsa Ranji, Univ. of Wisconsin-Milwaukee (United States)

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