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Journal of Biomedical Optics • Open Access

Dual-tracer background subtraction approach for fluorescent molecular tomography

Paper Abstract

Diffuse fluorescence tomography requires high contrast-to-background ratios to accurately reconstruct inclusions of interest. This is a problem when imaging the uptake of fluorescently labeled molecularly targeted tracers in tissue, which can result in high levels of heterogeneously distributed background uptake. We present a dual-tracer background subtraction approach, wherein signal from the uptake of an untargeted tracer is subtracted from targeted tracer signal prior to image reconstruction, resulting in maps of targeted tracer binding. The approach is demonstrated in simulations, a phantom study, and in a mouse glioma imaging study, demonstrating substantial improvement over conventional and homogenous background subtraction image reconstruction approaches.

Paper Details

Date Published: 4 January 2013
PDF: 12 pages
J. Biomed. Opt. 18(1) 016003 doi: 10.1117/1.JBO.18.1.016003
Published in: Journal of Biomedical Optics Volume 18, Issue 1
Show Author Affiliations
Kenneth M. Tichauer, Thayer School of Engineering at Dartmouth (United States)
Robert W. Holt, Dartmouth College (United States)
Fadi El-Ghussein, Thayer School of Engineering at Dartmouth (United States)
Scott C. Davis, Thayer School of Engineering at Dartmouth (United States)
Kimberley S. Samkoe, Dartmouth Hitchcock Medical Ctr. (United States)
Jason R. Gunn, Thayer School of Engineering at Dartmouth (United States)
Frederic Leblond, Thayer School of Engineering at Dartmouth (Canada)
Brian W. Pogue, Thayer School of Engineering at Dartmouth (United States)


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