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Journal of Biomedical Optics • Open Access

Understanding three-dimensional spatial relationship between the mouse second polar body and first cleavage plane with full-field optical coherence tomography
Author(s): Jing-gao Zheng; Tiancheng Huo; Tianyuan Chen; Chengming Wang; Ning Zhang; Ning Tian; Fengying Zhao; Dieyan Chen; Wanyun Ma; Jia-Lin Sun; Ping Xue; Danyu Lu

Paper Abstract

The morphogenetic relationship between early patterning and polarity formation is of fundamental interest and remains a controversial issue in preimplantation embryonic development. We use a label-free three-dimensional (3-D) imaging technique of full-field optical coherence tomography (FF-OCT) successfully for the first time to study the dynamics of developmental processes in mouse preimplantation lives. Label-free 3-D subcellular time-lapse images are demonstrated to investigate 3-D spatial relationship between the second polar body (2PB) and the first cleavage plane. By using FF-OCT together with quantitative study, we show that only 25% of the predicted first cleavage planes, defined by the apposing plane of two pronuclei, pass through the 2PB. Also only 27% of the real cleavage planes pass through the 2PB. These results suggest that the 2PB is not a convincing spatial cue for the event of the first cleavage. Our studies demonstrate the feasibility of FF-OCT in providing new insights and potential breakthroughs to the controversial issues of early patterning and polarity in mammalian developmental biology.

Paper Details

Date Published: 13 December 2012
PDF: 4 pages
J. Biomed. Opt. 18(1) 010503 doi: 10.1117/1.JBO.18.1.010503
Published in: Journal of Biomedical Optics Volume 18, Issue 1
Show Author Affiliations
Jing-gao Zheng, Tsinghua Univ. (China)
Tiancheng Huo, Tsinghua Univ. (China)
Tianyuan Chen, Tsinghua Univ. (China)
Chengming Wang, Tsinghua Univ. (China)
Ning Zhang, Tsinghua Univ. (China)
Ning Tian, Tsinghua Univ. (China)
Fengying Zhao, Tsinghua Univ. (China)
Dieyan Chen, Tsinghua Univ. (China)
Wanyun Ma, Tsinghua Univ. (China)
Jia-Lin Sun, Tsinghua Univ. (China)
Ping Xue, Tsinghua Univ. (China)
Danyu Lu, Peking Univ. (China)

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