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Journal of Biomedical Optics • Open Access

Sensing dynamic cytoplasm refractive index changes of adherent cells with quantitative phase microscopy using incorporated microspheres as optical probes
Author(s): Sabine Przibilla; Sebastian Dartmann; Angelika Vollmer; Steffi Ketelhut; Gert von Bally; Björn Kemper; Burkhard Greve

Paper Abstract

The intracellular refractive index is an important parameter that describes the optical density of the cytoplasm and the concentration of the intracellular solutes. The refractive index of adherently grown cells is difficult to access. We present a method in which silica microspheres in living cells are used to determine the cytoplasm refractive index with quantitative phase microscopy. The reliability of our approach for refractive index retrieval is shown by data from a comparative study on osmotically stimulated adherent and suspended human pancreatic tumor cells. Results from adherent human fibro sarcoma cells demonstrate the capability of the method for sensing of dynamic refractive index changes and its usage with microfluidics.

Paper Details

Date Published: 6 September 2012
PDF: 9 pages
J. Biomed. Opt. 17(9) 097001 doi: 10.1117/1.JBO.17.9.097001
Published in: Journal of Biomedical Optics Volume 17, Issue 9
Show Author Affiliations
Sabine Przibilla, Ctr. for Biomedical Optics and Photonics (Germany)
Sebastian Dartmann, Ctr. for Biomedical Optics and Photonics (Germany)
Angelika Vollmer, Ctr. for Biomedical Optics and Photonics (Germany)
Steffi Ketelhut, Ctr. for Biomedical Optics and Photonics (Germany)
Gert von Bally, Ctr. for Biomedical Optics and Photonics (Germany)
Björn Kemper, Ctr. for Biomedical Optics and Photonics (Germany)
Burkhard Greve, Univ. Hospital Munich (Germany)


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