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Journal of Biomedical Optics

Impact of the optical depth of field on cytogenetic image quality
Author(s): Yuchen Qiu; Yuhua Li; Hong Liu; Xiaodong Chen; Bin Zheng; Shibo Li; Wei R. Chen
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Paper Abstract

In digital pathology, clinical specimen slides are converted into digital images by microscopic image scanners. Since random vibration and mechanical drifting are unavoidable on even high-precision moving stages, the optical depth of field (DOF) of microscopic systems may affect image quality, in particular when using an objective lens with high magnification power. The DOF of a microscopic system was theoretically analyzed and experimentally validated using standard resolution targets under

Paper Details

Date Published: 1 September 2012
PDF: 7 pages
J. Biomed. Opt. 17(9) 096017 doi: 10.1117/1.JBO.17.9.096017
Published in: Journal of Biomedical Optics Volume 17, Issue 9
Show Author Affiliations
Yuchen Qiu, The Univ. of Oklahoma (United States)
Yuhua Li, The Univ. of Oklahoma (United States)
Hong Liu, The Univ. of Oklahoma (United States)
Xiaodong Chen, The Univ. of Oklahoma (United States)
Bin Zheng, Univ. of Pittsburgh (United States)
Shibo Li, The Univ. of Oklahoma Health Sciences Ctr. (United States)
Wei R. Chen, Univ. of Central Oklahoma (United States)


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