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Journal of Biomedical Optics

Mapping lipid and collagen by multispectral photoacoustic imaging of chemical bond vibration
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Paper Abstract

Photoacoustic microscopy using vibrational overtone absorption as a contrast mechanism allows bond-selective imaging of deep tissues. Due to the spectral similarity of molecules in the region of overtone vibration, it is difficult to interrogate chemical components using photoacoustic signal at single excitation wavelength. Here we demonstrate that lipids and collagen, two critical markers for many kinds of diseases, can be distinguished by multispectral photoacoustic imaging of the first overtone of C-H bond. A phantom consisting of rat-tail tendon and fat was constructed to demonstrate this technique. Wavelengths between 1650 and 1850 nm were scanned to excite both the first overtone and combination bands of C-H bonds. B-scan multispectral photoacoustic images, in which each pixel contains a spectrum, were analyzed by a multivariate curve resolution-alternating least squares algorithm to recover the spatial distribution of collagen and lipids in the phantom.

Paper Details

Date Published: 14 September 2012
PDF: 7 pages
J. Biomed. Opt. 17(9) 096010 doi: 10.1117/1.JBO.17.9.096010
Published in: Journal of Biomedical Optics Volume 17, Issue 9
Show Author Affiliations
Pu Wang, Purdue Univ. (United States)
Ping Wang, Purdue Univ. (United States)
Han-Wei Wang, Purdue Univ. (United States)
Ji-Xin Cheng, Purdue Univ. (United States)

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