Share Email Print

Journal of Biomedical Optics • Open Access

Optical mapping at increased illumination intensities
Author(s): Giedrius Kanaporis; Irma Martisiene; Jonas Jurevicius; Ruta Vosyliute; Antanas Navalinskas; Rimantas Treinys; Arvydas Matiukas; Arkady M. Pertsov

Paper Abstract

Voltage-sensitive fluorescent dyes have become a major tool in cardiac and neuro-electrophysiology. Achieving high signal-to-noise ratios requires increased illumination intensities, which may cause photobleaching and phototoxicity. The optimal range of illumination intensities varies for different dyes and must be evaluated individually. We evaluate two dyes: di-4-ANBDQBS (excitation 660 nm) and di-4-ANEPPS (excitation 532 nm) in the guinea pig heart. The light intensity varies from 0.1 to 5  mW/mm 2 , with the upper limit at 5 to 10 times above values reported in the literature. The duration of illumination was 60 s, which in guinea pigs corresponds to 300 beats at a normal heart rate. Within the identified duration and intensity range, neither dye shows significant photobleaching or detectable phototoxic effects. However, light absorption at higher intensities causes noticeable tissue heating, which affects the electrophysiological parameters. The most pronounced effect is a shortening of the action potential duration, which, in the case of 532-nm excitation, can reach ∼30% . At 660-nm excitation, the effect is ∼10% . These findings may have important implications for the design of optical mapping protocols in biomedical applications.

Paper Details

Date Published: 6 September 2012
PDF: 8 pages
J. Biomed. Opt. 17(9) 096007 doi: 10.1117/1.JBO.17.9.096007
Published in: Journal of Biomedical Optics Volume 17, Issue 9
Show Author Affiliations
Giedrius Kanaporis, Lithuanian Univ. of Health Sciences (Lithuania)
Irma Martisiene, Lithuanian Univ. of Health Sciences (Lithuania)
Jonas Jurevicius, Lithuanian Univ. of Health Sciences (Lithuania)
Ruta Vosyliute, Lithuanian Univ. of Health Sciences (Lithuania)
Antanas Navalinskas, Lithuanian Univ. of Health Sciences (Lithuania)
Rimantas Treinys, Lithuanian Univ. of Health Sciences (Lithuania)
Arvydas Matiukas, SUNY Upstate Medical Univ. (United States)
Arkady M. Pertsov, Lithuanian Univ. of Health Sciences (Lithuania)

© SPIE. Terms of Use
Back to Top